A new Buttiauxella phytase continuously hydrolyzes phytate and improves amino acid digestibility and mineral balance in growing pigs fed phosphorous-deficient diet作者：Zeng ZK, Li QY,Zhao PF, Xu X, Tian QY, Wang HL, Pan L, Yu SK and Piao XS*
时间: 2016 来源: 农业部饲料中心
期刊：J Anim Sci.2016.94:629-638
摘要：Ten ileal T-cannulated pigs (19.26±1.06 kg) were used to evaluate the effects of a novel Buttiauxella phytase on apparent ileal digestibility (AID) of AA and apparent total tract digestibility(ATTD) and hindgut disappearance of DM, GE, CP, crude fiber, NDF, and ADF as well as minerals balance. Pigs were fed in a duplicated 5 × 4 incomplete Latin square design (5 diets with 4 periods). Each period consisted of a 5-d adjustment period followed by a 3-d total collection of feces and urine and then a 2-d collection of ileal digesta. The 5 diets included a P-deficient basal diet(0.43% Ca and 0.38% total P) that was supplemented with 0 (negative control [NC]), 500, 1,000, or 20,000 phytase units (FTU)/kg phytase and a positive control (PC) diet that was P adequate (0.64% Ca and 0.52% total P). The addition of phytase to the NC diet improved(P < 0.05) AID of phytate from 11.1 to 62.8, 70.6, and 90.5% at the inclusion rates of 500, 1,000, and 20,000 FTU/kg, respectively. In general, phytase supplementation at a dose of 20,000 FTU/kg further increased (P <0.05) AID of Ca, total P, and phytate and reduced (P <0.05) the ileal phytate concentration compared with diets with 500 or 1,000 FTU/kg phytase. Pigs fed the diet with 20,000 FTU/kg phytase but not diets with 500 and 1,000 FTU/kg phytase showed improved (P < 0.05) ATTD of CP and AID of DM, GE, CP, Leu, Lys, Thr,Val, Asp, and Ser compared with pigs fed the PC or NC diet. However, hindgut disappearance of crude fiber and NDF (P < 0.05) were reduced in pigs fed the diet with 20,000 FTU/kg phytase compared with pigs fed the PC or NC diet. Pigs fed diets with 500 or 1,000 FTU/kg phytase had greater ATTD and retention of Ca and P than pigs fed the NC diet but less compared with pigs fed the diet with 20,000 FTU/kg phytase. Supplementation of 20,000 FTU/kg phytase to the NC diet improved (P <0.05) digestibility of Na, Mn, and Zn as well as retention(%) of Zn. Increasing phytase supplementation doses from 0 to 1,000 FTU/kg linearly improved (P <0.05) retention of Mg; meanwhle, digestibility of Mg and Mn and AID of Thr showed a linear increase trend (P = 0.084). In conclusion, supplementation of the novel Buttiauxella phytase at doses up to 20,000 FTU/kg hydrolyzed most of the phytate (90%) and cosequently further improved mineral and protein utilization.